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LabWits
Tools

Practical tools for laboratory workflows

Browse current LabWits tools by scientific workflow area. The library is organized around practical lab tasks and will continue to grow as new resources are added.

Cell Culture & Cell Biology

Planning aids for routine cell culture work, from seeding and dilution through growth tracking, banking, and manual counts.

Cell Seeding Calculator Calculate cell suspension and media volumes for a target seeding density across plates, wells, or culture vessels. Cell Dilution Calculator Calculate how much starting cell suspension and media are needed for a target concentration and final volume. Cell Doubling Time Calculator Estimate cell population doubling time from starting and final cell counts over a defined culture interval. Cell Growth Kinetics Calculator Evaluate cell growth rate, doubling behavior, and population expansion across culture measurements. Cell Freezing Calculator Calculate cryopreservation volume, DMSO amount, freezing density, and cryovial setup for cell banking workflows. Hemocytometer Cell Count Calculator Calculate cell concentration, viability, and total cell number from manual hemocytometer counts.

Viral & Lentiviral Workflows

Tools for common viral and lentiviral setup decisions where titer, cell number, and infection conditions need to align.

MOI Calculator Calculate virus volume from cell number, MOI, viral titer, and infection volume. Lentiviral Transduction Calculator Calculate lentiviral transduction mix volumes from cell number, target MOI, functional titer, and transduction volume.

Assay Analysis & Quality Control

Resources for interpreting assay signals, curve fits, control separation, and quality checks in everyday analysis workflows.

Percent Viability and Percent Inhibition Calculator Normalize assay signals to percent viability and percent inhibition with controls, quality checks, and graphing. IC50 / EC50 Calculator Fit normalized dose-response data with a 4PL model to estimate IC50 or EC50. Z-Factor / Z-Prime Calculator Calculate Z-factor and Z-prime from positive and negative assay controls.

Solution & Reagent Preparation

Preparation tools for dilutions, serial workflows, and reagent calculations that need clear bench-ready quantities.

C1V1 Dilution Calculator Solve C1V1 = C2V2 dilution calculations for stock concentration, target concentration, and preparation volume. Serial Dilution Calculator Plan serial dilution steps from a starting concentration to lower working concentrations. Molarity Calculator Calculate molarity, solute mass, molecular weight, or solution volume for reagent preparation.

Laboratory Instrument Utilities

Utilities for translating instrument settings and lab equipment parameters into practical working values.

RCF to RPM Calculator Convert between relative centrifugal force (RCF) and revolutions per minute (RPM) using rotor radius.

Molecular Biology

Support for PCR, qPCR, primer setup, nucleic-acid normalization, and related molecular biology preparation tasks.

Primer Tm Calculator Estimate primer melting temperature and basic sequence properties for PCR assay planning. Primer Resuspension and Dilution Calculator Calculate primer resuspension and working-stock dilution volumes from synthesis scale and target concentration. qPCR Relative Expression Calculator Calculate relative gene expression from qPCR Ct values using delta Ct and delta delta Ct workflows. PCR/qPCR Master Mix Calculator Calculate PCR and qPCR master mix volumes with overage, water adjustment, template handling, and bench-ready reagent tables. DNA/RNA Normalization Calculator Normalize DNA, RNA, cDNA, or other nucleic-acid samples to a shared target concentration. DNA Molecular Weight and Copy Number Calculator Calculate DNA molecular weight, molar amount, molar concentration, and estimated copy number from DNA length and mass or concentration.