Tools
Molecular Biology
Support for PCR, qPCR, primer setup, nucleic-acid normalization, and related molecular biology preparation tasks.
Primer Tm Calculator
Estimate primer melting temperature and basic sequence properties for PCR assay planning.
Primer Resuspension and Dilution Calculator
Calculate primer resuspension and working-stock dilution volumes from synthesis scale and target concentration.
qPCR Relative Expression Calculator
Calculate relative gene expression from qPCR Ct values using delta Ct and delta delta Ct workflows.
PCR/qPCR Master Mix Calculator
Calculate PCR and qPCR master mix volumes with overage, water adjustment, template handling, and bench-ready reagent tables.
DNA/RNA Normalization Calculator
Normalize DNA, RNA, cDNA, or other nucleic-acid samples to a shared target concentration.
DNA Molecular Weight and Copy Number Calculator
Calculate DNA molecular weight, molar amount, molar concentration, and estimated copy number from DNA length and mass or concentration.