#lv-qpcr-delta-delta-ct-calculator {
  font-family: var(--lv-font);
  color: var(--lv-navy);
  line-height: 1.5;
  background: #ffffff;
}

#lv-qpcr-delta-delta-ct-calculator *,
#lv-qpcr-delta-delta-ct-calculator *::before,
#lv-qpcr-delta-delta-ct-calculator *::after { box-sizing: border-box; }

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-hero {
  display: flex;
  align-items: center;
  justify-content: center;
  gap: 10px;
  margin: 8px auto 12px;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-hero > span {
  width: 42px;
  height: 1px;
  background: rgba(45, 108, 223, 0.28);
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-hero svg {
  width: 124px;
  height: auto;
  max-width: 38vw;
  flex: 0 0 auto;
}
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-hero rect { fill: #f8fbff; stroke: #d9e5f3; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-axis { stroke: #a9bacd; stroke-width: 1.3; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-threshold { stroke: #d59a27; stroke-dasharray: 4 3; stroke-width: 1.2; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-curve { fill: none; stroke-linecap: round; stroke-width: 2.4; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-curve-a { stroke: var(--lv-blue); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-curve-b { stroke: var(--lv-brand-dark); opacity: 0.7; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-ct { stroke: var(--lv-red-text); stroke-width: 1.4; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-hero text { fill: var(--lv-red-text); font-size: 8px; font-weight: 800; }
#lv-qpcr-delta-delta-ct-calculator .lv-input::placeholder {
  color: #9aa6b2;
  opacity: 1;
}
#lv-qpcr-delta-delta-ct-calculator .lv-select:invalid { color: #9aa6b2; }
#lv-qpcr-delta-delta-ct-calculator .lv-select option { color: var(--lv-navy); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-stack { display: grid; gap: 22px; }

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-setup-grid,
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-results-grid {
  display: grid;
  grid-template-columns: repeat(2, minmax(0, 1fr));
  gap: 14px;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-setup-grid { grid-template-columns: repeat(4, minmax(0, 1fr)); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-builder-actions { display: flex; flex-wrap: wrap; gap: 8px; margin: 6px 0 14px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-list { display: grid; gap: 8px; margin-bottom: 14px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row {
  display: grid;
  grid-template-columns: 110px minmax(180px, 1fr) auto;
  gap: 10px;
  align-items: start;
  padding: 10px 12px;
  border: 1px solid var(--lv-border);
  border-radius: 10px;
  background: var(--lv-bg);
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row > strong {
  padding-top: 11px;
  font-size: 13px;
}
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row > .lv-btn { margin-top: 8px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-labeled-field { min-width: 0; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-labeled-field .lv-label {
  display: block;
  margin-bottom: 5px;
}
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-labeled-field .lv-helper-text {
  margin-top: 5px;
  line-height: 1.35;
}
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-list { display: grid; gap: 12px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-card {
  border: 1px solid var(--lv-border);
  border-radius: 12px;
  background: #ffffff;
  overflow: hidden;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-card > summary {
  display: grid;
  grid-template-columns: 110px minmax(0, 1fr) auto auto;
  align-items: start;
  gap: 10px;
  padding: 14px;
  background: var(--lv-blue-soft);
  cursor: pointer;
  list-style: none;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-card > summary::-webkit-details-marker { display: none; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-target-label { padding-top: 11px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-target-input-wrap { min-width: 0; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-target-input-wrap .lv-helper-text { margin-top: 5px; line-height: 1.35; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-name { width: 100%; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-summary-label { padding-top: 11px; color: var(--lv-muted); font-size: 13px; font-weight: 700; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-remove-gene { margin-top: 8px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-body { display: grid; gap: 12px; padding: 14px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-sample-card {
  padding: 13px;
  border: 1px solid #dfe7f0;
  border-radius: 10px;
  background: #fbfdff;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-sample-card h4 { margin: 0 0 9px; font-size: 15px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-header,
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-row {
  display: grid;
  grid-template-columns: 92px minmax(0, 1fr) minmax(0, 1fr);
  gap: 9px;
  align-items: center;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-header {
  margin-bottom: 5px;
  color: var(--lv-muted);
  font-size: 12px;
  font-weight: 800;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-row + .lv-qpcr-replicate-row { margin-top: 7px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-row label { color: var(--lv-muted); font-size: 13px; font-weight: 700; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-entry-note { margin-top: 14px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-result { margin-bottom: 18px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-result h3 { margin-bottom: 9px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-table-wrap { width: 100%; overflow-x: auto; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-table { width: 100%; min-width: 1040px; border-collapse: collapse; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-table th,
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-table td { padding: 9px 8px; border-bottom: 1px solid #e5e7eb; text-align: left; vertical-align: top; font-size: 13px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-table th { background: var(--lv-bg); font-size: 12px; white-space: nowrap; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-interpretation { margin: 9px 0 0; color: var(--lv-muted); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-status {
  display: inline-block;
  padding: 3px 8px;
  border-radius: 999px;
  font-size: 12px;
  font-weight: 800;
  white-space: nowrap;
}

#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-status-good { color: var(--lv-green-text); background: #eefaf3; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-status-caution { color: var(--lv-yellow-text); background: #fff8e8; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-status-review { color: var(--lv-red-text); background: #fff1f1; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-panel { margin-top: 18px; padding: 16px; border: 1px solid var(--lv-border); border-radius: 12px; background: #fbfdff; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-panel h3 { margin-top: 0; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-list { display: grid; gap: 8px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-item { padding: 10px 12px; border-left: 4px solid #d7a32b; background: #fffaf0; border-radius: 6px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-item.lv-strong { border-left-color: var(--lv-red-text); background: #fff4f4; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-qc-item.lv-info { border-left-color: var(--lv-blue); background: var(--lv-blue-soft); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-graph-filter-wrap { max-width: 260px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-chart { display: block; width: 100%; min-width: 460px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-chart text { fill: var(--lv-muted); font-family: var(--lv-font); font-size: 11px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-chart-grid { stroke: #dfe6ef; stroke-width: 1; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-chart-bar { fill: var(--lv-blue); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-chart-control { fill: var(--lv-brand-dark); }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-summary-card { display: grid; gap: 7px; }
#lv-qpcr-delta-delta-ct-calculator .lv-qpcr-summary-card p { margin: 0; }
#lv-qpcr-delta-delta-ct-calculator .lv-meta-section { max-width: 1100px; margin: 34px auto 8px; padding-top: 18px; border-top: 1px solid #e5e7eb; }
#lv-qpcr-delta-delta-ct-calculator .lv-meta-section p { margin: 0; color: #7a8491; font-size: 13px; }

@media (max-width: 900px) {
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-setup-grid { grid-template-columns: repeat(2, minmax(0, 1fr)); }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-results-grid { grid-template-columns: 1fr; }
}

@media (max-width: 620px) {
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-setup-grid,
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row { grid-template-columns: 1fr; }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row > strong,
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-condition-row > .lv-btn { margin-top: 0; padding-top: 0; }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-header { display: none; }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-replicate-row { grid-template-columns: 1fr; gap: 5px; padding-top: 9px; border-top: 1px solid #e5e7eb; }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-gene-card > summary { grid-template-columns: 1fr; }
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-target-label,
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-summary-label,
  #lv-qpcr-delta-delta-ct-calculator .lv-qpcr-remove-gene { margin-top: 0; padding-top: 0; }
}